Evaluation of antimicrobial sensitivity and resistance pattern of Gram positive and Gram negative bacteria to monitor drug resistance among clinical isolates
نویسندگان
چکیده
Introduction: Antibiotic therapy is usually given to protect from infection against various organisms, however, routine use of empirical treatment has resulted in widespread antibiotic resistance and development of antibiotic resistant genes. Objective: To identify the bacterial pathogens isolated from various clinical specimens and to determine their antibiotic sensitivity and resistance pattern against the commonly used standard antibiotics. Materials & Methods: All the clinical specimens submitted to microbiology laboratory were processed as per standard microbiological procedures and antibiotic sensitivity tests were performed on the isolates as per guidelines of clinical and laboratory standards institute (CLSI). Result: A total of 154 organisms were isolated from all clinical specimens, out of which 82 were Gram positive and 72 were Gram negative bacteria. Doxycycline (63.4%) and amikacin (84.4%) showed high degree of sensitivity among Gram positive bacteria. Amikacin (77.8%) showed high degree of sensitivity in Gram negative bacteria. Gram positive bacteria showed highest degree of resistance to penicillin (63.4%), whereas, Gram negative bacteria showed highest degree of resistance to cefazolin (73.6%). Conclusion: High frequency of resistance against commonly used antibiotics such as penicillin and cefazolin as shown in the present study indicates a serious problem in the treatment of infections by gram positive and negative organisms. Therefore continuous surveillance is needed and treatment based on antibiogram report is essential.
منابع مشابه
Frequency of Multi-Drug Resistance (MDR) in Gram Negative Bacteria from Urinary Infection in Gorgan, 2011-12
Abstract Background and objective: The periodic evaluation of antimicrobial activity of different antibiotic is essential because antibiotic sensitivity pattern may also changed during short courses. The aim of this study was to assess the frequency of Multi-drug Resistance (MDR) in Gram negative uropathogens. Material and Methods: This study was conducted on 111 gram negative uropatho...
متن کاملDetermination of the Resistance Pattern of Prevalent Aerobic Bacterial Infections of Diabetic Foot Ulcer
Background and Objective: Diabetes mellitus is one of the main problems in health systems in the world. Diabetic Foot infections (DFI) is one of the main complications and the most cause of non-traumatic lower limb amputation .This study aimed to determine the prevalence of bacteria involved in DFI and their antibiotic resistance in patients with DFI diagnosis. Patients and Metho...
متن کاملPrevalence and Antibiotic Resistance Pattern of Extended Spectrum β-Lactamase- (ESBL-) Producing Gram Negative Bacilli isolated from Clinical Samples of hospitalized Patients in a hospital in Gorgan
Background & Aim: Antibiotic resistance among gram-negative bacilli is currently one of the major challenges for the health system in developing countries. The aim of this study was to determine the frequency and antibiotic resistance pattern of common ESBL-producing gram negative bacilli isolated from clinical specimens of hospitalized patients in Falsafi Hospital in Gorgan. Methods: In this ...
متن کاملMultidrug resistance pattern of bacterial agents isolated from patient with chronic sinusitis
Background: Treatment of chronic sinusitis is complicated due to increase of antibiotic-resistant bacteria. The aim of this study was to determine the multidrug resistance (MDR) pattern of the bacteria causing chronic sinusitis in north of Iran. Methods: This cross-sectional study was carried out on patients with chronic sinusitis. Bacterial susceptibility to antimicrobial agents was determi...
متن کاملAntibiotic Resistance Pattern of Gram-Negative Bacteria in Gorgan
Abstract Background and Objective: The excessive use of broad-spectrum antibiotics will lead to drug resistance of microorganism and specially nosocomial organisms. Because of high incidence of antibiotic resistance in hospitals, we aimed to study antibiotic resistance to gram negative bacteria. Material and Methods: This cross-sectional study was conducted on the data of biological sampl...
متن کامل